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ZymoPURE II Plasmid Purification

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ZymoPURE II

Plasmid Purification Reinvented.


See how simple plasmid purification could be.

Simple Workflow

Don't wait for gravity.

Supplier Q

ZymoPURE II

Supplier Q

ZymoPURE II

Midi & Maxipreps in less than 20 minutes

ZymoPURE II is plasmid purification reinvented. At the core of our ZymoPURE II technology is a novel binding chemistry and membrane that redefines plasmid purity, reduces processing time by 7 fold, and enables > 1 mg of plasmid to be eluted from a microcentrifuge column.

Elution means Elution.

Tired of performing tedious alcohol precipitations? With ZymoPURE II, the plasmid DNA is bound onto a spin-column and all of the contaminants are washed away prior to eluting in a microcentrifuge. The resulting eluate is transfection-ready and highly concentrated, so there is no need to remove salts and concentrate the plasmid after elution. Be confident in your elution with ZymoPURE.

Good-bye Gravity Flow.

Say farewell to the drip. Your time is valuable, so why spend it waiting for gravity? With ZymoPURE II, the plasmid DNA is bound onto a spin-column in mere seconds using a vacuum manifold or centrifuge, which completely eliminates the need for slow gravity flow columns.

Elute from a Spin Column

Load on Vacuum

No Ethanol Precipitation

Highest Yields

Tiny but mighty.


More Plasmid

Minimum Elution Volume

Consistently elute ≥ 1 µg/µl

Highly Concentrated Eluates

Novel ZymoPURE II binding technology results in 6 times more DNA using 65 times less matrix.

Yield and concentration for plasmid DNA isolated using the ZymoPURE™ II Maxiprep kit compared to two separate kits from Supplier Q. Plasmid DNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate).

Plasmid DNA yield and concentration from the ZymoPure™ II Maxiprep kit compared to other major suppliers. Plasmid DNA (pGEM®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).

Transfection Grade

EndoZero. Demand Zero or Nothing.

Endotoxins below FDA Limit for Vaccines

Sensitive Transfection Ready

In Vivo Ready

ZymoPURE II has set the new standard for purity. Are you sure endotoxins are not affecting your results? Why take the risk? Eliminate any chance of adverse effects with a simple spin.

Redefine Purity with EndoZero Plasmid DNA

Stated manufacturers’ endotoxin levels for the ZymoPURE™ II Maxiprep kit compared to two separate kits from Supplier Q.

Confident and Consistent Handling

See it to believe it.

Visualize complete lysis and neutralization for easy, error free preparation.

Patented Multi-Colored Buffer System

Unrivaled Technology

The easy choice.



ZymoPURE II Maxiprep Kit Supplier Q Endofree Maxiprep Supplier Q Maxiprep
Maximum Yield 1,200 µg 500 µg 500 µg
Endotoxins ≤ 0.025 EU/µg ≤ 0.1 EU/µg ≤ 10 EU/µg
Time per Prep < 20 min 150 min 160 min
Hands-On Time 15 min 45 min 45 min
Load via Vacuum or Centrifuge (No Gravity Flow)
Elute from a Spin-Column (No Precipitation)
Vaccine Grade*
In Vivo & Gene Therapy Grade
Transfection Grade (Sensitive & Primary Cells)

* Endotoxins below the FDA limit for Vaccines ( < 0.04 EU/µg of plasmid DNA)

Suitable for Any Application

Cited for sensitive techniques.

Transfection CRISPR
Lentivirus Production Adenovirus Production
shRNA siRNA
Cell-Free Protein Synthesis Generate Transgenic Organisms
Site-Directed Mutagenesis Microinjection of Embryos
Sequencing PCR
Cloning In Vitro Transcription
Highest Customer Satisfaction

Unforgettable Experiences.

"Overall, the kit is really easy to use, quick and the yield was the best I have ever obtained from a midi"

- H.H., MIT

“I love it. Easy to use, good yield. I WILL definitely replace my Q kit with this”

- K.J., Mayo Clinic School of Medicine

“It was very easy to use, taking hours off my prep time. The yield was good, 363 µg & 516 µg from 50 ml cultures, and the quality of DNA was also good. I wish I had more of these kits right now!”

- M.V. University of Pennsylvania

“This kit is exactly what our lab
has been looking for”

- H.M., Washington University in St. Louis

“This kit is pretty great, midiprep DNA in about the time it takes to do a miniprep.”

- J.G., Yale University


Customer data demonstrates that isolated plasmid DNA is transfection-ready

Data generated by V.B. at University of Cologne. HeLa cells seeded in a 6-well plate were transfected with either 2 or 4 µg of pCI-neo® + GFP plasmid isolated from 100 ml of bacteria culture using the ZymoPURE™ Midiprep kit, ZymoPURE™ Maxiprep kit, or NucleoBond® Xtra Midi kit from Macherey-Nagel. GFP expression was assesed 48 hours later in cell lysates using western blot and Ponceau S staining. The blot was also probed with an antibody against alpha-Tubulin in order to verify equal loading of samples.

Plasmid Purification Reinvented

Processing Volume Processing Time Yield Elution Volume Endotoxins Instruction Manual
ZymoPURE Plasmid Miniprep Kit ≤ 5 ml ≤ 15 min ≤ 100 µg ≥ 25 µl ≤ 1 EU/µg DNA
ZymoPURE II Plasmid Midiprep Kit ≤ 50 ml ≤ 20 min ≤ 400 µg ≥ 100 µl ≤ 0.025 EU/µg DNA
ZymoPURE II Plasmid Maxiprep Kit ≤ 150 ml ≤ 20 min ≤ 1.2 mg ≥ 200 µl ≤ 0.025 EU/µg DNA
ZymoPURE II Plasmid Gigaprep Kit ≤ 2.5 L ≤ 50 min ≤ 10 mg ≥ 2 ml ≤ 0.025 EU/µg DNA


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