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    Zyppy-96 Plasmid Kit

    D4041 / D4042 / D4043


    Zyppy-96 Plasmid Kit

    Cat # Name Size
    D4041 Zyppy-96 Plasmid Kit 2 x 96 Preps
    D4042 Zyppy-96 Plasmid Kit 4 x 96 Preps
    D4043 Zyppy-96 Plasmid Kit 8 x 96 Preps
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    Highlights
    • Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
    • High Quality: Ready for PCR, sequencing, cloning, and transfection.
    • Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
    Description

    The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.

    Applicable For Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
    Elution Volume ≥ 30 µl
    Equipment Centrifuge with microplate carriers with height tolerance of 60 mm (2.36 inches)
    Processing Time 45 min
    Purity Typical Abs 260/280 ≥ 1.8.
    Size Range Up to 25 kb
    Supplemental Info
    Yield Up to 5 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed.

    No

    10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

    Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.

    No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.

    ≤ 50 Endotoxin Units/µg of plasmid DNA.

    Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4°C as soon as possible.

    Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.

    Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.

    No, the sediment inside the buffer is insoluble, but it needs to be completely resuspended in the buffer prior to use for optimal performance.

    Cat # Name Size
    D4036-5-60 Zyppy Elution Buffer 60 ml
    D4041-1-30 Deep Blue Lysis Buffer 30 ml
    D4041-1-48 Deep Blue Lysis Buffer 48 ml
    D4041-4-100 Neutralization/Clearing Buffer 100 ml
    D4041-4-200 Neutralization/Clearing Buffer 200 ml
    D4036-5-30 Zyppy Elution Buffer 30 ml
    D4036-5-100 Zyppy Elution Buffer 100 ml
    D4036-4-48 Zyppy Wash Buffer (Concentrate) 48 ml
    D4036-4-24 Zyppy Wash Buffer (Concentrate) 24 ml
    D4036-3-160 Endo-Wash Buffer 160 ml
    D4036-3-120 Endo-Wash Buffer 120 ml
    C2007-24 96-Well Plate Cover Foil 24 Foils
    C2007-6 96-Well Plate Cover Foil 6 Foils
    C2011-8 Air Permeable Sealing Cover 8 Pack
    C2011-4 Air Permeable Sealing Cover 4 Pack
    C2011-2 Air Permeable Sealing Cover 2 Pack
    C2007-12 96-Well Plate Cover Foil 12 Foils
    C2004 Zymo-Spin I-96 Plate 2 Plates
    P1001-2 96-Well Block 2 Blocks
    C2002 Collection Plate 2 Plates
    C2003 Elution Plate 2 Plates

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