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    Quick-DNA Tissue/Insect 96 Kit

    D6017


    Quick-DNA Tissue/Insect 96 Kit

    Cat # Name Size
    D6017 Quick-DNA Tissue/Insect 96 Kit 2 x 96 Preps
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    Highlights
    • Simple, high-throughput (96-well) method for efficient isolation of DNA from insects, including mosquitoes, bees, lice, ticks, and D. melanogaster .
    • Compatible with tough-to-lyse tissues from other organisms.
    Description

    The Quick-DNA Tissue/Insect 96 Kit is an insect DNA extraction kit designed for the simple, high-throughput (96-well) method for the rapid isolation of DNA (e.g., genomic, viral, mitochondrial) from fresh, frozen, or stored insect specimens including mosquitoes, bees, lice, ticks, and D. melanogaster . The procedure is easy and can be completed in minutes: samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. The DNA is then isolated and purified using our Zymo-Spin technology and is ideal for downstream molecular-based applications including PCR, array, genotyping, etc. The procedure is compatible with mammalian tissues, whole blood, and cultured cells.

    Applicable For All sensitive downstream applications such as qPCR and Next-Generation sequencing.
    Elution Volume ≥ 25 µl
    Equipment Centrifuge with microplate carriers, 96-well plate/block disruptor or pulverizer.
    Purity A260/A280 nm ≥1.8.
    Sample Source Insect or solid tissue
    Sample Storage DNA stored at ≤ -20°C.
    Size Range Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.
    Supplemental Info
    Type Total DNA
    Yield 5 µg total DNA

    A viscous sample can indicate incomplete sample lysis. Try using less of your sample and optimize bead beating conditions (duration, speed, time) to ensure samples are thoroughly lysed. After bead beating, pellet the cell debris before moving on. Adding more Genomic Lysis buffer to the lysate can help dilute and deproteinate the sample, making the sample less viscous and more suitable for DNA recovery.

    Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.

    No additional RNase A treatment is required when processing samples within kit capacity. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through.

    Cat # Name Size
    S6002-96-2 ZR-96 BashingBead Lysis Rack 2 mm
    C2003 Elution Plate 2 Plates
    C2002 Collection Plate 2 Plates
    C2001 Silicon-A Plate 2 Plates
    P1001-2 96-Well Block 2 Blocks
    C2007-4 96-Well Plate Cover Foil 4 Foils
    D6001-3-40 BashingBead Buffer 40 ml
    D3004-5-50 DNA Pre-Wash Buffer 50 ml
    D3004-4-10 DNA Elution Buffer 10 ml
    D3004-2-100 g-DNA Wash Buffer 100 ml
    D3004-1-150 Genomic Lysis Buffer 150 ml

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